The work based on modulation of nano Schiff base ligand by using pharmacologically important compounds (indoline-2,3-dione) and aniline. Complextion of the prepared Schiff base and (1,10 phenanthroline or TMEDA) as mixed ligand with different transtion metal ions were carried out. This nano ligand and its nano binary and mixed metal complexes show two novel features; (1) an effective metal ion chelator and (2) it can play a pivot as antimicrobial and anticancer agent.